Transfection calculator

DailyCalcs turns your phone into a science calculator to simplify everyday tasks in the lab. Eight calculators, including a new Specific Productivity Calculator for use with fed-batch cell culture processes and a Transfection calculator you can use to simplify transfection experimental setup.The Specific Productivity Calculator for fed-batch cell culture processes takes into account Duration of Culture (Days), VCD and Titer levels to calculate early and late qP results. Next step recommendations are suggested based on the calculations, with an in-app ability to email your results for consultation assistance.The Transfection calculator addresses the transfection reagent, number of samples, the vessel size, and number of replicates and it calculates the exact amounts of all necessary reagents and media for your experiment.This application features the 8 following calculators absolutely free:1. Molarity CalculatorThe Molarity Calculator tool will allow you to find the mass required to prepare a solution of known volume & concentration, find the volume of solution required to dissolve a known mass to a specific molarity or find a concentration of a solution resulting from a known mass & volume.2. Dilution CalculatorThe Dilution Calculator tool will allow you to determine the required volume of a stock solution of known concentration to make a final solution of desired volume & concentration. This tool greatly simplifies the everyday tasks of making solutions in the lab.3. Molecular Weight CalculatorThe Molecular Weight Calculator will compute the average molecular weight (MW) of molecules by entering in the chemical formula (i.e. C3H2O4). Supports complex molecules such as "C3H2(NO)4" & "C3H2Cl4.(H2O)3" & is linked into the Molarity Calculator to work in tandem for molarity calculations.4. Cell Culture Reference ChartsReference charts for cell culture dishes, plates & flasks showing vital data such as growth surface area, cell seeding density, number of cells at confluency, volume of growth media necessary & required versene or trypsin volume for cell detachment.5. Unit ConverterA comprehensive unit converter tool capable of converting over 44 units in 7 different types of measurements including the following:Distance converter (m, ft, in, cm, mm, yards & km)Speed converter (m/sec, ft/sec, in/sec, cm/sec, km/sec, miles/sec, miles/hr, km/hr, knots)Area converter (m2, ft2, in2, yd2, acres, km2, miles2)Volume converter (liters, m3, cm3, ft3, in3, yards3)Weight converter (kg, g, oz, lbs, tons)Temperature converter (Celcius, Farenheit, Kelvin)Molar converter (mole, mM, μmol, nmol, pmol, fmol).6. Conversions for MediaUnit of measure conversion tool for powder and AGT media that converts Gibco powder and AGT media products between KG and L.7. Specific Productivity CalculatorThe Specific Productivity Calculator for fed-batch cell culture processes takes into account Duration of Culture (Days), VCD and Titer levels to calculate early and late qP results. Next step recommendations are suggested based on the calculations, with an in-app ability to email your results

System Lipofectamine 3000 reagent Xenon Electroporation System Thermo Fisher Scientific offers a complete collection of transfection reagents with exceptional performance for the delivery of DNA, siRNA, Invitrogen Stealth RNAi, and Invitrogen BLOCK-iT RNAi vectors, in traditional or difficult-to-transfect cell lines. Refer to the Seven Steps to RNAi Success when planning RNAi experiments.Use the Transfection Protocols & Citations tool to find the RNAi and siRNA protocols that you need, and explore Lipofectamine RNAiMAX reagent for advanced and efficient delivery of siRNA (see general transfection protocol using Lipofectamine RNAiMAX reagent). Additional information on RNAi and siRNA transfection protocols Stealth RNAi and siRNA transfection concentrations The transfection concentration of a Stealth RNAi or siRNA duplex is determined by dividing the number of moles of siRNA used by the final volume of the transfection (i.e. starting medium volume + transfection mixture volume). Using a 24-well plate, we typically transfect 0.5-5 pmol of siRNA in a 100 µL transfection mix to 500 µL of medium in each well to give a 1 to 10 nM final concentration. If transfecting in triplicate, make 400 µL of 5x transfection mix by adding 0.1–1 µL of the 20 µM stock to 399 µL of Gibco Opti-MEM. Then add 100 µL of this transfection mix to each of your wells. (20 µM Stealth RNAi or siRNA = 20 pmol/µL) Co-transfecting a vector and siRNA When co-transfecting plasmids and siRNA, use the appropriate plasmid transfection protocol. The siRNA component usually adds negligible nucleic acid mass and can be ignored. Find appropriate transfection protocols for plasmid vector transfection using the Transfection Protocols & Citations tool. Neon Electroporation System transfection protocols The Neon Transfection System offers an innovative electroporation method, helping you achieve high transfection efficiency in difficult-to-transfect cells, including immune, primary, and stem cells.Using the Transfection Protocols & Citations tool, find Neon Electroporation System-specific protocols. These protocols include general information about the cell line or primary cell, electroporation parameters used, transfection efficiency and survival rate achieved, and brightfield and fluorescence microscopy images of the cells following electroporation. For Research Use Only. Not for use in diagnostic procedures.

We’ve consolidated our extensive library of cell transfection protocols and citations into a single, easy-to-use tool. Narrow the results by cell type, cell line, payload, transfection product, or type of document. You’ll see a list of protocols and their efficiencies. What types of cell transfection protocols can I find in the tool? The transfection protocols and citations tool contains resources specific to a multitude of cell lines and types, including HeLa cells, CHO cells, Jurkat cells, and many others. Protocols and citations have also been categorized according to desired product (e.g., Lipofectamine or Neon electroporation system) and payload, allowing you to easily narrow your search to the cell culture transfection protocol most applicable to your experiment. Cell line-specific transfection protocols Find protocols that have been optimized for efficiency, viability, and reproducibility in your cell line. Cell lines available in this tool include, but are not limited to, the following.Cell typeSourceA549 Human lung carcinoma ACHN Human renal cancer C6 Rat glioma Caco-2 Human colorectal adenocarcinoma CHO-K1 Hamster ovary COS-7 Monkey kidney fibroblast D3 Mouse embryonic stem cells HCT-116 Human colon carcinoma HEK 293 Human embryonic kidney HeLa Human cervical carcinoma HepG2 Human liver carcinoma HL60 Human lymphoblast HT29 Human colon adenocarcinoma HT-1080 Human fibrosarcoma HUVEC Human umbilical vein cord IMR-90 Human embryonic lung Jurkat Human T-cell leukemia K-562 Human myelogenous leukemia LNCap Human prostate cancer MCF-7 Human breast cancer MDA-MB-231 Human breast adenocarcinoma MDA-MB-435 Human breast adenocarcinoma Molt-4 Human T lymphoblast NCI-H460 Human large cell lung cancer NHFF Human diploid somatic fibroblast NIH-3T3 Mouse embryonic fibroblast NTera2 Human embryonic carcinoma stem cell PC-3 Human prostate adenocarcinoma PC12 Rat neuronal SK-BR3 Human breast carcinoma SK-MEL-28 Human malignant melanoma SK-OV-3 Human ovarian adenocarcinoma THP-1 Human monocytic leukemia Product-specific transfection protocols Transfection products from Thermo Fisher Scientific are designed for highly efficient and reproducible results. Explore protocols designed for use with specific products, including:AAV-MAX transfection kit Lipofectamine CRISPRMAX reagent Expi293 Expression System Lipofectamine LTX reagent ExpiCHO Expression System Lipofectamine MessengerMAX reagent ExpiSF Expression System Lipofectamine RNAiMAX reagent FreeStyle MAX Reagent Lipofectamine Stem reagent Invivofectamine 3.0 reagent LV-MAX transfection kit Lipofectamine 2000 reagent Neon Transfection